Pharmacological Investigation on the Wound Healing Effects of Glycyrrhizic Acid Ammonium Salt in Rats

Abstract

Glycyrrhizic acid is triterpenoid saponin found in Glycyrrhiza glabra. Chemically, glycyrrhizin is sulphated polysaccharide .It is considered to the active constituents of the drug and the standardization of liquorice is based on glycyrrhizin content (Singh 2005). has been shown to possess ulcer healing activity. The present work with GA was undertaken with the premise that the drug promoting ulcer healing could have effect on wound healing also. Wound healing process involves several steps, which involves coagulation, inflammation, formation of granulation tissue, matrix formation, remodeling of connective tissue, collagenisation and aquisation of wound strength.Wound healing activity of GA was Glycyrrhizic acid ammonium salt (GA) studied in terms of 1) % wound contraction, 2) epithelization, 3) wound breaking strength in incision and excision wound models. In the standard group the rats were treated with 0.0005% w/w fluticasone ointment for 10 days in incision model and for 16 days in excision model. In the test group, the rats were treated with 2% w/w GA ointment for 10 days in incision model and for 16 days in excision model. In this study we have demonstrated the effect of glycyrrhizic acid ammonium salt on excision and incision wound healing models that GA show increase the % wound contraction, epithelization and wound breaking strength as compared to control group