Polymorphismus im leptin-gen in verbindung mit ausgewählten reproduktions-leistungen von ebern

Abstract

Studies on the porcine genome contributed to identification of polymorphic loci of the genes that may influence the level of reproduction-related traits. The LEP gene codes for leptin - a protein secreted into the blood from adipocytes, important for food intake, energy expenditure, and immune response; the protein also influences some functions of the mammalian reproductive system. The aim of this study was to determine the polymorphism of HinfI in the leptin gene and its effect on selected qualitative and quantitative traits of semen. The analyses included 238 AI boars. The genotypes were established with PCR-RFLP. Two alleles LEP/HinfI were identified: LEP T (152 bp) and LEP C (84 and 68hp) with the frequencies 0.8445 and 0.1555 respectively. The genotype frequencies were as follows: LEP TLEP T - 0.7227, LEP CLEP T - 0.2437, LEP CLEP C - 0.0336. The present study showed that LEP TLEP T boars produced ejaculates of larger volume, higher percentage, number of sperm in ejaculate and number of insemination doses.