Spectrophotometric Estimation of Gemcitabine in bulk drug and its Formulation

Abstract

Gemcitabine is a pyramidine analog which is used in the treatment of cancer. Three simple, sensitive, accurate and economical spectrophotometric methods have been developed for the estimation of Gemcitabine in bulk drug and pharmaceutical formulation. Method A is based on UV spectrophotometric measurements in which Gemcitabine was dissolved in double distilled water and exhibited absorption maximum at 266 nm and obeyed Beers law in the concentration range of 5-30 g/ml. Method B is based on measurements of first order derivative spectroscopy adopted to eliminate spectral interference, in which derivative amplitude was measured at 260.5 nm. Method C is based on calculation of area under curve for analysis of Gemcitabine in wavelength range of 260-271 nm. The drug obeys Beers law in the concentration range of 5-30 µg/ml. Results of the analysis were validated for accuracy, precision, Limit of Detection (LOD), Limit of Quantification (LOQ) were found to be satisfactory. The proposed methods are simple, sensitive, rapid, economical, and suitable for the routine quality control application in pharmaceutical formulations